The user can select from a list of all open images. A dialog window opens, asking for some information: Image: The image which should be displayed in the viewer. Histogram equalization may have an impact but for your image it doesn’t appear to be appropriate. After you have started the 3D viewer, click on File Add content. Most automatic thresholding is independent of such histogram scaling. Here are three histograms of your grayscale image:Ĭontrast enhanced with histogram equalizationĪs you may realize, the first two histograms have the same shape. Please remenber that neither thresholding nor using the wand tool generalize well!įinally, I don’t think that contrast enhancement is helpful because most criteria for finding the threshold won’t be affected, except if histogram equalization is checked. Understanding the processing steps and carefully planning of your project are essential for a reasonable processing and analysis of more than a single image. ![]() These stacks are images of cells and I want to only colocalize signal within them, ignoring all signal outside. Please carefully study the ImageJ-manual.įurthermore, please consider the general recommendations that I made earlier. Hi all Looking for some advice about getting any of the ImageJ plugins to play ball for colocalization of largeish z-stacks (130 slices). However, I suspect that you don’t really understand what you are doing and what the various steps really mean. What you show in your latest post equals my result.
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